Next Generation Sequencing

High-throughput technologies and instruments used in our facility 

PacBio Sequel DNA sequencer uses Single Molecule Real Time (SMRT) technology to produce long reads, uniform coverage, and high consensus accuracy. The Sequel long 10-15kb reads are ideal for whole genome sequencing, full-length transcript sequencing, or sequencing of long amplicons. Additionally, its SMRT sequencing technology can also be used to directly detect DNA base modifications.

• Whole genome and targeted re-sequencing
• De novo genome sequencing
• SNP, CNV and INDEL Detection
• Cancer Panels
• ChIP-Seq and exo ChIP-Seq
• Methylome discovery
• Whole transcriptome sequencing
• Exome Sequencing
• Small RNA sequencing
• SOLiD/Illumina fragment, paired-end and mate-pair library
• Illumina HiSeq 2500 sequencing (in collaborating facility)
• SAGE

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Genomic Data Analysis

Genomic Data Analysis service available from our facility. We have extensive experience in identification, annotation, prediction, De Novo and resquencing projects with large datasets.

• Variant (SNP & Indel) and Structural Variation Discovery
• Exome Sequencing Analysis
• Complete RNA-seq analysis including Differential Gene Expression, gene fusion and Novel Isoform (Alternative Splicing) Identification
• Small and miRNA Sequencing Analysis
• ChIP-Seq Peak Analysis
• De Novo Genome & Transcriptome Assembly
• Genome Finishing
• 16S rRNA Metagenomics Analysis

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