The Waksman Institute Shared Imaging Facility is located on Busch Campus of Rutgers, The State University of New Jersey. Our Facility provides training and access to cutting edge optical imaging capabilities on five fluorescent microscopes and two image analysis workstations and provides researchers the ability to image a variety of sample types. Some of the microscope features include deconvolution, quantification, co-localization, FRET, FRAP, FLIM, FCS, time-lapse, spinning disk super resolution (SRRF-Stream), photo-ablation optogenetics, structured illumination super resolution (SIM, SMLM and TIRF) and SIM widefield (WF), as well as software tools for image analysis. We are open to researchers from Rutgers University as well as from other institutions and companies in the area. Our services include consultation for instrument selection, training, technical support, individual use by trained and approved researchers, and consultation on image analysis. We do not provide sample prep services. 

Overview of Instruments 

• Leica TCS SP8 WLL Confocal Microscope with Fluorescence Recovery After Photobleaching (FRAP)

  • Lasers: 405, Argon (458, 476, 488, 496, 514) and White Light Laser (WLL) 470-670nm

• Leica TCS SP8 Lng Confocal Microscope with Lightning Deconvolution

  • Lasers: 405, 488, 552 and 638nm

• Leica TCS Stellaris8 Confocal Microscope with White Light Laser (WLL), TauSense, Falcon FLIM, FRET-FLIM, Fluorescence Correlation Spectroscopy (FCS), and Lightning Deconvolution

  • Lasers: 405nm fixed and pulsed Extreme White Light Laser 440-790nm

• Andor Dragonfly Spinning Disk Microscope with SRRF-Stream Super Resolution, MOSAIC FRAP and photo stimulation, MicroPoint ablation, deconvolution, and environmental chamber

  • Lasers: 405, 488, 514, 561, 637 and 685nm

• Zeiss Elyra7 Lattice Structured Illumination Microscope (SIM2) with Super Resolution Single Molecule Localization Microscopy (SMLM), Total Internal Reflection Microscopy (TIRF), Widefield (WF) imaging, and environmental chamber. 

  • Lasers: 405, 488, 561 and 640nm

• Zeiss workstation with Zen software and remote access

• Leica workstation with LAS X software