Protocols

Duckweeds are ideal material for physiological, biochemical, and genomic studies because of their direct contact with medium, rapid growth and relatively small genome sizes. They are valuable means for biomanufacturing through genetic engineering technology and due to the recent progress towards duckweed-based commercial products. They can be easily maintained by vegetative reproduction in aseptic cultivation for decades. The small size of the plant is ideal for maintaining diverse accessions and therefore for evolutionary studies at the DNA level. Some species, such as Lemna minor, are used by the Environmental Protection Agency for measuring water quality because their growth rates are sensitive to a wide range of environmental contaminants such as metals, nitrates, and phosphates. Rapid growth also offers practical applications of duckweeds as a biofuel crop. It is becoming basic skills to maintain, grow and DNA bardode them in duckweed research community. Here we present the details in the following links. 

 

Methods

Protocol of growing duckweeds

Protocol of total genomic DNA isolation from duckweeds with CTAB

Protocol of DNA barcode duckweeds by atpF-atpH marker