Publications

2013
Klimuk, E, Akulenko N, Makarova KS, Ceyssens P-J, Lavigne R, Severinov K.  2013.  Host RNA polymerase inhibitors encoded by φKMV-like phages of Pseudomonas. Virology. 436:67-74.
2012
Severinov, K, Nair S.  2012.  The action of microcin C and mechanisms of bacterial resistance to it. Future Microbiol. 7:281-289.
Westra, ER, van Erp PB, Wong SP, Kunne T, Staals R, Seegers CL, Bollen S, Jore MM, de Vos WM, Dame RT et al..  2012.  CRISPR immunity relies on the conservative binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3. Mol. Cell. 46:595-605.
Djordjevic, M, Djordjevic M, Severinov K.  2012.  CRISPR transcript processing: an unusual mechanism for strong amplification of small RNAs. Biol. Direct. 7:24.
Glukhov, AS, Krutilina AI, Shlyapnikov MG, Severinov K, Lavysh D, Kochetkov VV, McGrath JW, de Leeuwe C, Shaburova OV, Krylov VN et al..  2012.  Genomic analysis of Pseudomonas putida phage tf with localized single-strand DNA interruptions. PLoS One. 7:e51163.
Berdygulova, Z, Esyunina D, Miropolskaya N, Mukhamedyarov D, Kuznedelov K, Nickels B, Severinov K, Kulbachinskiy A, Minakhin L.  2012.  The gp39 protein of phage P23-45 is a transcription antiterminator that acts by suppressing pausing by Thermus thermophilus RNA polymerase. Nucl. Acids Res. 40:4052-4063.
Shadrin, A, Sheppard C, Matthews S, Severinov K, Wigneshweraraj S.  2012.  Inhibition of transcription initiation by T7 Gp2 occurs through at least two different sets of interactions with the E. coli RNA polymerase. Microbiology SGM. 158:2753-2764.
Datsenko, KA, Pougach K, Tikhonov A, Wanner BL, Severinov K, Semenova E.  2012.  Prior encounters dramatically stimulate adaptive bacterial CRISPR immune response to viruses. Nature Commun. 3:945.
Mekler, V, Minakhin L, Kuznedelov K, Mukhamedyarov D, Severinov K.  2012.  Species-specific interactions determining different stability of promoter complexes formed by RNA polymerases from Escherichia coli and Thermus aquaticus. Nucl. Acids Res. 40:11352-11362.
Nocek, B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondehhoff G, Van Aershot A, Anderson W, Severinov K et al..  2012.  Structural and functional characterization of microcin C resistance peptidase MccF from Bacillus anthracis. J. Mol. Biol. 420:366-383.
James, E, Liu M, Sheppard C, Mekler V, Camara B, Liu B, Simpson P, Cota E, Severinov K, Matthews S et al..  2012.  Structural basis for the inhibition of the Escherichia coli RNA polymerase by T7 Gp2. Molecular Cell. 47:755-776.
Agarwal, V, Tikhonov A, Metlytskaya A, Severinov K, Nair S.  2012.  Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis inhibitor Microcin C7. Proc. Natl. Acad. Sci. USA. 109:4425-4430.
Pavlova, O, Lavysh D, Klimuk E, Djordjevic M, Ravcheev DA, Gelfand MS, Severinov K, Akulenko N.  2012.  Temporal regulation of gene expression of the Escherichia coli bacteriophage phiEco32. J. Mol. Biol. 416:389-399.
Pougach, K, Severinov K.  2012.  Use of semi-quantitative Northern blot analysis to determine relative quantities of bacterial CRISPR transcripts. Methods in Molecular Biology on Bacterial Regulatory RNA . :73-86.
2011
Kuznedelov, K, Semenova E, Knappe T, Marahiel M, Ebright RHE, Severinov K.  2011.  Antibacterial threaded lasso-peptide capistruin is a DNA-dependent RNA polymerase inhibitor. J. Mol. Biol. 412:842-848.
Severinov, K, Semenova E, Kazakov T.  2011.  Class I microcins: Their structures activities, and mechanisms of resistance. Prokaryotic Antimicrobial Peptides: from Genes to Applications. :289-308.
Vondenhoff, GHM, Dubiley S, Severinov K, Lescrinier E, Rozenski J, Van Aerschot A.  2011.  Extended targeting potential and improved synthesis of Microcin C analogues as antibacterials. Bioorg. & Med. Chem.. 19:5462-5467.
Semenova, E, Jore MM, Datsenko KA, Semenova A, Westra ER, Wanner B, van der Oost J, Brouns SJ, K. S.  2011.  Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence.. Proc Natl Acad Sci U S A.. 108(25):10098-103.
Ghilarov, D, Serebryakova M, Shkundina I, Severinov K.  2011.  A major portion of microcin B17 undergoes an N,O-peptidyl shift during synthesis.. J. Biol. Chem.. 286:26308-26318.
Mekler, V, Minakhin L, Sheppard C, Wigneshweraraj S, Severinov K.  2011.  Molecular mechanism of transcription inhibition by phage T7 gp2 protein.. J. Mol. Biol.. 413:1016-1027.
Mekler, V, Minakhin L, Severinov K.  2011.  On the role of downstream RNA polymerase-promoter interactions in formation of transcription initiation complex.. J. Biol. Chem.. 286:22600-22608.
Nagornykh, M, Zakharova M, Protsenko A, Bogdanova E, Solonin A, Severinov K.  2011.  The regulation of gene expression in the Eco29kI restriction-modification system. . Nucleic Acids Res.. 39:4653-4663.
Yuzenkova, Y, Severinov K, Zenkin N.  2011.  The role of the β’ zipper element in promoter recognition. . EMBO J.. 30:3766-3775.