DNA barcoding of the Lemnaceae, a family of aquatic monocots

Wang, W, Wu Y, Yan Y, Ermakova M, Kerstetter R, Messing J.  2010.  

Journal:

BMC Plant Biol

Volume Number:

10

Pages:

205

Abstract:

BACKGROUND: Members of the aquatic monocot family Lemnaceae (commonly called duckweeds) represent the smallest and fastest growing flowering plants. Their highly reduced morphology and infrequent flowering result in a dearth of characters for distinguishing between the nearly 38 species that exhibit these tiny, closely-related and often morphologically similar features within the same family of plants. RESULTS: We developed a simple and rapid DNA-based molecular identification system for the Lemnaceae based on sequence polymorphisms. We compared the barcoding potential of the seven plastid-markers proposed by the CBOL (Consortium for the Barcode of Life) plant-working group to discriminate species within the land plants in 97 accessions representing 31 species from the family of Lemnaceae. A Lemnaceae-specific set of PCR and sequencing primers were designed for four plastid coding genes (rpoB, rpoC1, rbcL and matK) and three noncoding spacers (atpF-atpH, psbK-psbI and trnH-psbA) based on the Lemna minor chloroplast genome sequence. We assessed the ease of amplification and sequencing for these markers, examined the extent of the barcoding gap between intra- and inter-specific variation by pairwise distances, evaluated successful identifications based on direct sequence comparison of the "best close match" and the construction of a phylogenetic tree. CONCLUSIONS: Based on its reliable amplification, straightforward sequence alignment, and rates of DNA variation between species and within species, we propose that the atpF-atpH noncoding spacer could serve as a universal DNA barcoding marker for species-level identification of duckweeds.

Notes:

Wang, WenqinWu, YongruiYan, YihengErmakova, MarinaKerstetter, RandallMessing, JoachimResearch Support, Non-U.S. Gov'tEnglandBMC plant biologyBMC Plant Biol. 2010 Sep 16;10:205.

Related External URL:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20846439
Citation:
Wang, W, Wu Y, Yan Y, Ermakova M, Kerstetter R, Messing J.  2010.  DNA barcoding of the Lemnaceae, a family of aquatic monocots. BMC Plant Biol. 10:205.