Multiple Barriers to Non-homologous DNA End Joining During Meiosis in Drosophila

Joyce, EF, Paul A, Chen KE, McKim KS.  2012.  

Journal:

Genetics

Volume Number:

191

Pages:

739-46

Abstract:

Repair of meiotic double-strand breaks (DSBs) uses the homolog and recombination to yield crossovers while alternative pathways such as nonhomologous end-joining (NHEJ) are suppressed. Our results indicate that NHEJ is blocked at two steps of DSB repair during meiotic prophase: first by the activity of the MCM-like protein MEI-218 that is required for crossover formation and, second, by Rad51-related proteins SPN-B (XRCC3) and SPN-D (RAD51C) that physically interact and promote homologous recombination. We further show that the MCM-like proteins also promote the activity of the DSB repair checkpoint pathway, indicating an early requirement for these proteins in DSB processing. We propose that when a meiotic DSB is formed in the absence of both MEI-218 and SPN-B or SPN-D, a DSB substrate is generated that can enter the NHEJ repair pathway. Indeed, due to its high error rate, multiple barriers may have evolved to prevent NHEJ activity during meiosis.

Notes:

Journal article

Related External URL:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22542963
Citation:
Joyce, EF, Paul A, Chen KE, McKim KS.  2012.  Multiple Barriers to Non-homologous DNA End Joining During Meiosis in Drosophila. Genetics. 191:739-46.