Spectinomycin resistance mutations in the <i>rrn16</i> gene are new plastid markers in <i>Medicago</i> <i>sativa</i>

Dudas, B, Jenes B, Kiss GB, Maliga P.  2012.  

Journal:

Theor. Appl. Genet

Volume Number:

125

Pages:

1517-23

Abstract:

We report here the isolation of spectinomycin-resistant mutants in cultured cells of Medicago sativa line RegenSY-T2. Spectinomycin induces bleaching of cultured alfalfa cells due to inhibition of protein synthesis on the prokaryotic type 70S plastid ribosomes. Spontaneous mutants resistant to spectinomycin bleaching were identified by their ability to form green shoots on plant regeneration medium containing selective spectinomycin concentrations in the range of 25-50 mg/l. Sequencing of the plastid rrn16 gene revealed that spectinomycin resistance is due to mutations in a conserved stem structure of the 16S rRNA. Resistant plants transferred to the greenhouse developed normally and produced spectinomycin-resistant seed progeny. In light of their absence in soybean, a related leguminous plant, the isolation of spectinomycin-resistant mutants in M. sativa was unexpected. The new mutations are useful for the study of plastid inheritance, as demonstrated by detection of predominantly paternal plastid inheritance in the RegenSY-T2 x Szapko57 cross, and can be used as selective markers in plastid transformation vectors to obtain cisgenic plants.

Notes:

Dudas, BrigittaJenes, BarnabasKiss, Gyorgy BotondMaliga, PalGermanyTheor Appl Genet. 2012 Nov;125(7):1517-23. doi: 10.1007/s00122-012-1930-7. Epub 2012 Jul 13.

Related External URL:

http://www.ncbi.nlm.nih.gov/pubmed/22791077
Citation:
Dudas, B, Jenes B, Kiss GB, Maliga P.  2012.  Spectinomycin resistance mutations in the rrn16 gene are new plastid markers in Medicago sativa. Theor. Appl. Genet. 125:1517-23.