Genome-wide association of Yorkie with chromatin and chromatin-remodeling complexes

Oh, H, Slattery M, Ma L, Crofts A, White KP, Mann RS, Irvine KD.  2013.  


Cell reports

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The Hippo pathway regulates growth through the transcriptional coactivator Yorkie, but how Yorkie promotes transcription remains poorly understood. We address this by characterizing Yorkie's association with chromatin and by identifying nuclear partners that effect transcriptional activation. Coimmunoprecipitation and mass spectrometry identify GAGA factor (GAF), the Brahma complex, and the Mediator complex as Yorkie-associated nuclear protein complexes. All three are required for Yorkie's transcriptional activation of downstream genes, and GAF and the Brahma complex subunit Moira interact directly with Yorkie. Genome-wide chromatin-binding experiments identify thousands of Yorkie sites, most of which are associated with elevated transcription, based on genome-wide analysis of messenger RNA and histone H3K4Me3 modification. Chromatin binding also supports extensive functional overlap between Yorkie and GAF. Our studies suggest a widespread role for Yorkie as a regulator of transcription and identify recruitment of the chromatin-modifying GAF protein and BRM complex as a molecular mechanism for transcriptional activation by Yorkie.


Oh, HyangyeeSlattery, MatthewMa, LijiaCrofts, AlexWhite, Kevin PMann, Richard SIrvine, Kenneth D3U01HG004264/HG/NHGRI NIH HHS/5P50GM081892/GM/NIGMS NIH HHS/5R01GM054510/GM/NIGMS NIH HHS/GM078620/GM/NIGMS NIH HHS/P50 GM081892/GM/NIGMS NIH HHS/R01 GM054510/GM/NIGMS NIH HHS/R01 GM078620/GM/NIGMS NIH HHS/U01 HG004264/HG/NHGRI NIH HHS/Howard Hughes Medical Institute/Cell Rep. 2013 Feb 21;3(2):309-18. doi: 10.1016/j.celrep.2013.01.008. Epub 2013 Feb 8.

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Oh, H, Slattery M, Ma L, Crofts A, White KP, Mann RS, Irvine KD.  2013.  Genome-wide association of Yorkie with chromatin and chromatin-remodeling complexes. Cell reports. 3:309-18.