Structural Basis of Transcription Initiation.

Zhang, Y, Feng Y, Chatterjee S, Tuske S, Ho MX, Arnold E, Ebright RH.  2012.  

Journal:

Science (New York, N.Y.)

Volume Number:

338

Issue Number:

6110

Pages:

1076-1080

Abstract:

During transcription initiation, RNA polymerase (RNAP) binds and unwinds promoter DNA to form an RNAP-promoter open complex. We have determined crystal structures at 2.9 and 3.0 Å resolution of functional transcription initiation complexes comprising Thermus thermophilus RNA polymerase, σ(A), and a promoter DNA fragment corresponding to the transcription bubble and downstream dsDNA of the RNAP-promoter open complex. The structures show that σ recognizes the -10 element and discriminator element through interactions that include the unstacking and insertion into pockets of three DNA bases, and that RNAP recognizes the -4/+2 region through interactions that include the unstacking and insertion into a pocket of the +2 base. The structures further show that interactions between σ and template-strand ssDNA preorganize template-strand ssDNA to engage the RNAP active center.

Related External URL:

http://www.sciencemag.org/cgi/rapidpdf/338/6110/1076?ijkey=FEW7wKK5L0XoA&keytype=ref&siteid=sci
Citation:
Zhang, Y, Feng Y, Chatterjee S, Tuske S, Ho MX, Arnold E, Ebright RH.  2012.  Structural Basis of Transcription Initiation.. Science (New York, N.Y.). 338(6110):1076-1080.