About the Facility

The Waksman Confocal Microscope Core Facility provides training and consultation on the use of our confocal microscopes. The facility has two Leica TCS confocal microscopes, the SP8 and SP5 II, as well as a workstation. Our scanning confocal microscopes are capable of high resolution imaging of labeled cell components in three dimensional space by optical sectioning.  Imaging can be done with most standard fluorophores in live or fixed samples. 

Confocal microscopes

Both Leica TCS confocal models have

  • Inverted microscope
  • Spectral detection allowing for dynamic adjustment of detected emission wavelengths
  • Sequential scanning to further reduce emission signal overlap
  • Easy to use programs for creating Z-stacks and 3-D images
  • Multiple programs for post-imaging

Leica TCS SP5 II

  • Objectives: 10x dry, 20x immersion, 40x oil, 63x oil, 100x oil
  • Lasers: 405 Diode laser for UV, Argon (458, 476, 488, 496, 514), 543, 594, and 633
  • Two state of the art Hybrid detectors for large dynamic range, increased signal sensitivity and decreased background noise, as well as two standard PMT detectors
  • A motorized stage allowing multiple regions of the sample to be monitored over time, as well as Mark and Find and Tile Scanning.
  • Resonant Scanner
  • Z-stack compensation

  Leica TCS SP8

  • Objectives: 10x dry, 20 x oil, 40x oil, 63x oil
  • Lasers: 405, 488, 552, and 638
  • Two state of the art Hybrid detectors for large dynamic range, increased signal sensitivity and decreased background noise, as well as three standard PMT detectors.
  • Z-stack compensation
  • Improved 3D software

 

The facilities are primarily for use by Waksman Institute researchers. Depending upon availability and approval by the Confocal Manager, outside researchers may be able to use the facilities. Anyone interested in using the facility should Contact Us.

Services available include:

  • User training
  • User assistance and machine troubleshooting