Differential expression of a gene for a methionine-rich storage protein in maize

Kirihara, JA, Hunsperger JP, Mahoney WC, Messing JW.  1988.  

Journal:

Molecular & general genetics : MGG

Volume Number:

211

Pages:

477-84

Abstract:

A methionine-rich 10 kDa zein storage protein from maize was isolated and the sequence of the N-terminal 30 amino acids was determined. Based on the amino acid sequence, two mixed oligonucleotides were synthesized and used to probe a maize endosperm cDNA library. A full-length cDNA clone encoding the 10 kDa zein was isolated by this procedure. The nucleotide sequence of the cDNA clone predicts a polypeptide of 129 amino acids, preceded by a signal peptide of 21 amino acids. The predicted polypeptide is unique in its extremely high content of methionine (22.5%). The maize inbred line BSSS-53, which has increased seed methionine due to overproduction of this protein, was compared to W23, a standard inbred line. Northern blot analysis showed that the relative RNA levels for the 10 kDa zein were enhanced in developing seeds of BSSS-53, providing a molecular basis for the overproduction of the protein. Southern blot analysis indicated that there are one or two 10 kDa zein genes in the maize genome.

Notes:

0026-89252452963

Related External URL:

http://www.ncbi.nlm.nih.gov/sites/entrez?db=PubMed&cmd=retrieve&dopt=AbstractPlus&list_uids=2452963file://localhost/Users/messing/Documents/Lett.&Memos/New%20Text/Current%20Papers/Papers/1988/Kirihara/KiriharaMol%20Gen%20Genet%201988.pdfpapers://E07DE6F9-
Citation:
Kirihara, JA, Hunsperger JP, Mahoney WC, Messing JW.  1988.  Differential expression of a gene for a methionine-rich storage protein in maize. Molecular & general genetics : MGG. 211:477-84.