<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kim, T K</style></author><author><style face="normal" font="default" size="100%">Ebright, R. H.</style></author><author><style face="normal" font="default" size="100%">Reinberg, D</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mechanism of ATP-dependent promoter melting by transcription factor IIH.</style></title><secondary-title><style face="normal" font="default" size="100%">Science (New York, N.Y.)</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Science</style></alt-title></titles><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000 May 26</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">288</style></volume><pages><style face="normal" font="default" size="100%">1418-22</style></pages><abstract><style face="normal" font="default" size="100%">We show that transcription factor IIH ERCC3 subunit, the DNA helicase responsible for adenosine triphosphate (ATP)-dependent promoter melting during transcription initiation, does not interact with the promoter region that undergoes melting but instead interacts with DNA downstream of this region. We show further that promoter melting does not change protein-DNA interactions upstream of the region that undergoes melting but does change interactions within and downstream of this region. Our results rule out the proposal that IIH functions in promoter melting through a conventional DNA-helicase mechanism. We propose that IIH functions as a molecular wrench: rotating downstream DNA relative to fixed upstream protein-DNA interactions, thereby generating torque on, and melting, the intervening DNA.</style></abstract><issue><style face="normal" font="default" size="100%">5470</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/10827951?dopt=Abstract</style></custom1></record></records></xml>